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RESEARCH PRODUCT
The expression level of GCNF affects fate choice during neural differentiation of PCC7 cells
Christina ZechelUlrike G. A. SattlerIngrid Koziollek-drechslersubject
GeneticsTransgeneRetinoic acidRegulatorCell BiologyBiologymedicine.diseaseEmbryonic stem cellCell biologyEmbryonal carcinomachemistry.chemical_compoundchemistryNuclear receptorSense (molecular biology)medicineMolecular BiologyPsychological repressiondescription
The nuclear receptor GCNF (NR6A1) is required for embryonic survival and development, and regulation of fertility. We used a transgenic approach to investigate its role in neural differentiation. As model we chose the embryonal carcinoma cell line PCC7, which reproducibly differentiates into a tissue-like pattern of neuronal and non-neuronal cells after exposure to retinoic acid (RA). The differentiation pattern of gcnf sense and antisense clones consistently indicated that the expression level of GCNF positively correlated with the development of the neuronal fate. Moreover, antisense clones failed to down-regulate expression of the key regulator of differentiation Oct4 during the initial stages of neural differentiation. This data proposes that GCNF might promote the neuronal fate by mechanisms that involve repression of Oct4.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-04-01 | Signal Transduction |