6533b82afe1ef96bd128c19f
RESEARCH PRODUCT
Sidelobe decline in single-photon 4Pi microscopy by Toraldo rings.
Pedro AndrésManuel Martínez-corralMaría Teresa Caballero CaballeroA. Ponssubject
Point spread functionMicroscopeMaterials sciencePhotonMicroscopy ConfocalSuper-resolution microscopybusiness.industryConfocalGeneral Physics and AstronomyCell BiologyModels Theoreticallaw.inventionOpticsImaging Three-DimensionalStructural Biologylaw3d imageMicroscopyGeneral Materials Science4Pi microscopebusinessFiltrationdescription
We demonstrate theoretically the feasibility of single-photon 4Pi-confocal microscopy. By inserting a pair of properly designed multi-ring phase-only pupil filters in the illumination path of a 4Pi microscope the height of the sidelobes of the point spread function substantially reduced, so that there is no ambiguity in the 3D image. Then, an axial resolution up to four times higher than that of single-photon confocal microscope can be effectively achieved.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-08-23 | Micron (Oxford, England : 1993) |