6533b82afe1ef96bd128c388
RESEARCH PRODUCT
Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery
Vincenzo CerulloMatti JalasvuoriTiia A. TurunenTapani ViitalaMikko P. TurunenMarjo YliperttulaAndres LõhmusHeikki SaariSarah J. ButcherSeppo Ylä-herttualaPia Siljandersubject
MECHANISM0301 basic medicineOncolytic adenovirusHistologyadenoviruHEPATITIS-B-VIRUSGenetic enhancementvirusesTETRASPANINGene deliveryBiologysolukalvotGENE DELIVERYPATHWAY03 medical and health sciences0302 clinical medicineImmune systemlcsh:QH573-671MICROVESICLESEXOSOMESsyöpähoidotlcsh:CytologyMICROPARTICLESadenoviruksetCell BiologyadenovirusExtracellular vesiclesVirologyMicrovesicles3. Good healthOncolytic virus030104 developmental biologyLytic cycle030220 oncology & carcinogenesisCELLSCancer cellonkolyyttiset virukset1182 Biochemistry cell and molecular biologycancer therapyAUTOPHAGYonkolyyttinen virushoitoextracellular vesiclesResearch ArticleDNA deliverydescription
Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell-derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of infected cells. IEVs were able to carry the viral genome and induce infection in other cancer cells. As such, the role of EVs in the life cycle of adenoviruses may be an important part of a successful infection and may also be harnessed for cancer- and gene therapy. Peer reviewed
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-01-01 |