0000000000668982

AUTHOR

Pia Siljander

showing 3 related works from this author

Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery

2020

Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell-derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of…

MECHANISM0301 basic medicineOncolytic adenovirusHistologyadenoviruHEPATITIS-B-VIRUSGenetic enhancementvirusesTETRASPANINGene deliveryBiologysolukalvotGENE DELIVERYPATHWAY03 medical and health sciences0302 clinical medicineImmune systemlcsh:QH573-671MICROVESICLESEXOSOMESsyöpähoidotlcsh:CytologyMICROPARTICLESadenoviruksetCell BiologyadenovirusExtracellular vesiclesVirologyMicrovesicles3. Good healthOncolytic virus030104 developmental biologyLytic cycle030220 oncology & carcinogenesisCELLSCancer cellonkolyyttiset virukset1182 Biochemistry cell and molecular biologycancer therapyAUTOPHAGYonkolyyttinen virushoitoextracellular vesiclesResearch ArticleDNA delivery
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Biological properties of extracellular vesicles and their physiological functions

2015

The authors wish to thank Dr R Simpson and Dr D Taylor for critical reading of the manuscript and acknowledge the Horizon 2020 European Cooperation in Science and Technology programme and its support of our European Network on Microvesicles and Exosomes in Health & Disease (ME-HaD; BM1202 www.cost.eu/COST_Actions/bmbs/Actions/BM1202). In the past decade, extracellular vesicles (EVs) have been recognized as potent vehicles of intercellular communication, both in prokaryotes and eukaryotes. This is due to their capacity to transfer proteins, lipids and nucleic acids, thereby influencing various physiological and pathological functions of both recipient and parent cells. While intensive invest…

ProteomicsCellular distributionMATURE DENDRITIC CELLSReviewReview ArticleUrineEmbryo developmentMonocyteProtein processingVascular biologyFecesVesícules seminalsSYNCYTIOTROPHOBLAST MICROVILLOUS MEMBRANESCell selectionPregnancyT lymphocyteBileCELL-DERIVED EXOSOMESBiogenesisLung lavageUterus fluidInnate immunityMale genital systemlcsh:CytologyMicrovesicleOUTER-MEMBRANE VESICLESBlood clottingprokaryoteEukaryotaExtracellular vesicleRNA analysisCell biologyBloodCerebrospinal fluidLiver metabolismmicrovesicleMorphogenHumanNervous systemCell signalingBreast milkNatural killer cellFisiologiaExtracellular vesiclesExosomelcsh:QH573-671SalivaBiologyBiology and Life SciencesDNAPlantRNA transportCell functionMacrophageMolecular biologyPhysiologyMedizinProteomicsFACTOR PATHWAY INHIBITOReukaryoteProtein glycosylationExtracellular spaceTissue repairEspai extracel·lularReticulocyteSeminal plasmaMesenchymal stem cellAntigen presenting cellSeminal vesiclesNose mucusBiofilmNeutrophilMicroRNAPLANT-MICROBE INTERACTIONSLipidAmnion fluidProkaryotamicroparticleCell interactionCell transporteukaryote exosome extracellular vesicle microparticle microvesicle physiology prokaryoteBone mineralizationMicroorganismHistologyAdaptive immunityMembrane vesicleComputational biologyMembrane receptorBiologyStressCell communicationMast cellMESENCHYMAL STEM-CELLSHUMAN ENDOTHELIAL-CELLSexosomeCytokineSynovial fluidCell BiologyNonhumanIMMUNE-MODULATORY FEATURESReview articleDNA contentphysiologyRNAINTESTINAL EPITHELIAL-CELLSextracellular vesicleBody fluidLectinBiogenesis
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Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine

2016

collaboration au projet H2020 European Cooperation in Science and Technology (COST) program European Network on Microvesicles and Exosomes in Health and Disease (ME-HAD); International audience; Recent research has demonstrated that all body fluids assessed contain substantial amounts of vesicles that range in size from 30 to 1000 nm and that are surrounded by phospholipid membranes containing different membrane microdomains such as lipid rafts and caveolae. The most prominent representatives of these so-called extracellular vesicles (EVs) are nanosized exosomes (70-150 nm), which are derivatives of the endosomal system, and microvesicles (100-1000 nm), which are produced by outward budding…

0301 basic medicineMedical nanotechnologyPhysiologyMedizinGeneral Physics and Astronomyxxx xxxCell CommunicationExosomesRegenerative medicineTheranostic NanomedicineMembrane microparticleEngineering (all)Drug Delivery SystemsPathophysiologicalCell-Derived MicroparticlesCaveolaeDiagnosisGeneral Materials ScienceLipid raftPhospholipidsClinical Trials as TopicPhospholipid membraneVesicleGeneral EngineeringScience and TechnologyEngineering (all); Materials Science (all); Physics and Astronomy (all)3. Good healthCell biologyIntercellular communicationsClinical trial (topic)NanomedicineDrug deliveryRegenerative medicine[SDV.IMM]Life Sciences [q-bio]/ImmunologyNanomedicineMaterials Science (all)HumanEndosomeDrug delivery systemNanotechnologyBiologyProgram diagnosticsPhysics and Astronomy (all)03 medical and health sciencesExtracellular VesiclesAnimalsHumansTherapeutic agentsSettore BIO/16 - Anatomia UmanaAnimalRecent researchesMicrovesiclesCell membranesExosome030104 developmental biologyInternational cooperationMembrane microdomains
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