6533b82afe1ef96bd128cbd4

RESEARCH PRODUCT

Revisiting the thiosemicarbazonecopper(II) reaction with glutathione. Activity against colorectal carcinoma cell lines.

Carlos García-girónKatia G. SamperJoaquín BorrásTeófilo RojoFriederike I. NollmannMaría S. GalleteroJavier García-tojalVíctor Ivo FouzLuis LezamaÒScar PalaciosPilar MuñizGotzon MadariagaMónica Cavia-saizRubén Gil-garcíaRaquel Alcaraz

subject

Aparato digestivo-EnfermedadesThiosemicarbazonesSpectrometry Mass Electrospray IonizationColorectal cancerColon carcinoma010402 general chemistryCrystallography X-RayThiosemicarbazone01 natural sciencesBiochemistryInorganic Chemistrychemistry.chemical_compoundColon carcinomaCell Line TumorSpectroscopy Fourier Transform InfraredmedicineHumansMolecular magnetismDigestive organs-DiseasesMolecular Structure010405 organic chemistryChemistryMyoglobinCytochromes cGlutathioneChemistry Inorganicmedicine.diseaseMolecular biologyGlutathioneQuímica inorgánica0104 chemical sciencesCell cultureDrug Screening Assays AntitumorColorectal NeoplasmsCopper

description

Thiosemicarbazones (TSCs), and their copper derivatives, have been extensively studied mainly due to the potential applications as antitumor compounds. A part of the biological activity of the TSC-CuII complexes rests on their reactivity against cell reductants, as glutathione (GSH). The present paper describes the structure of the [Cu(PTSC)(ONO2)]n compound (1) (HPTSC =pyridine-2-carbaldehyde thiosemicarbazone) and its spectroscopic and magnetic properties. ESI studies performed on the reaction of GSH with 1 and the analogous [{Cu (PTSC*)(ONO2)}2] derivative (2, HPTSC* =pyridine-2-carbaldehyde 4N-methylthiosemicarbazone) show the absence of peaks related with TSC-Cu-GSH species. However GSH-Cu ones are detected, in good agreement with the release of CuI ions after reduction in the experimental conditions. The reactivity of 1 and 2 with cytochrome c and myoglobin and their activities against HT-29 and SW-480 colon carcinoma cell lines are compared with those shown by the free HPTSC and HPTSC* ligands.

yearjournalcountryeditionlanguage
2018-03-01Journal of inorganic biochemistry
10.1016/j.jinorgbio.2017.12.005https://pubmed.ncbi.nlm.nih.gov/29247869