6533b82bfe1ef96bd128ce07

RESEARCH PRODUCT

Pulsed electric field (PEF) recovery of biomolecules from Chlorella: Extract efficiency, nutrient relative value, and algae morphology analysis

Min WangJianjun ZhouJuan Manuel CastagniniHouda BerradaFrancisco J. Barba

subject

MineralsChlorophyll AChlorellaNutrientsGeneral MedicinePulsed electric fields (PEF)AntioxidantsAnalytical ChemistryAlguesSolventsMicroalgaeAlgues d'aigua dolçaDimethyl SulfoxideNutritive ValueMicrostructureFood Science

description

This study investigated the effects of pulsed electric field (PEF) (3 kV/cm, 44 pulses, 99 kJ/kg), solvent (H2O or 50 % DMSO) and time (0, 10, 20, 30, 60, 90, 120 and 180 min) on the extraction of Chlorella antioxidant biomolecules and minerals. The results showed that PEF treatment increased the biomolecules recovery. For the extraction time of 120 min, more proteins and polyphenols were obtained using water, while more chlorophyll a and b, and carotenoids were obtained using 50 % DMSO as the extraction solvent. The extracts mineral concentration (PEF vs control) were analysed including Mg, P, Ca, Fe and Zn, and the Relative Nutrient Values results indicated that Chlorella H2O-extracts could be used as a mineral source for different populations. Finally, the fluorescence and scanning electron microscopy revealed the electroporation effect of PEF.

yearjournalcountryeditionlanguage
2023-01-01Food Chemistry
https://doi.org/10.1016/j.foodchem.2022.134615