6533b82bfe1ef96bd128ce57
RESEARCH PRODUCT
Zein as biodegradable material for effective delivery of alkaline phosphatase and substrates in biokits and biosensors
Elizabeth A. H. HallPilar Campíns-falcóN. Jornet-martínezsubject
media_common.quotation_subjectZeinBiomedical EngineeringBiophysicsBiocompatible Materials02 engineering and technologyBiosensing Techniques01 natural sciencesPlasticizersGratitudeAbsorbable ImplantsAlkaline phosphataseElectrochemistrySalivamedia_commonChemistry010401 analytical chemistryfood and beveragesPhosphorusGeneral MedicineEquipment Design021001 nanoscience & nanotechnologyBiomaterial0104 chemical sciencesEquipment Failure AnalysisPesticideSpectrometry FluorescenceBiosensorsBiochemistryAbsorption PhysicochemicalAlkaline phosphataseColorimetryReagent Kits Diagnostic0210 nano-technologyBiotechnologydescription
A biodegradable material, zein, is proposed as a reagent delivery platform for biokits and biosensors based on alkaline phosphatase (ALP) activity/inhibition in the presence of phosphatase substrates. The immobilization and release of both the substrate and/or the active ALP, in a biodegradable and low-cost material such as zein, a prolamin from maize, and in combination with glycerol as plasticizer have been investigated. Three zein-based devices are proposed for several applications: (1) inorganic phosphorus estimation in water of different sources (river, lake, coastal water and tap water) with a detection limit of 0.2mg/L - compared to at least 1mg/L required by legislation, (2) estimation of ALP in saliva and (3) chlorpyrifos control in commercial preparations. The single-use kits developed are low cost, easy and fast to manufacture and are stable for at least 20 days at -20°C, so the zein film can preserve and deliver both the enzyme and substrates.
year | journal | country | edition | language |
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2016-04-09 |