6533b82bfe1ef96bd128d51e
RESEARCH PRODUCT
Immunological detection of tonoplast polypeptides in the plasma membrane of pea cotyledons
Hans-peter HaschkeGiselbert HinzFrancis MartyMasayoshi MaeshimaBirgit HohDavid Robinsonsubject
0106 biological sciences0303 health sciencesInorganic pyrophosphatasePyrophosphataseATPasefood and beveragesPlant ScienceImmunogold labellingVacuoleBiologybiology.organism_classification01 natural sciencesProton pumpPisumBlot03 medical and health scienceschemistry.chemical_compoundBiochemistrychemistryGeneticsbiology.protein030304 developmental biology010606 plant biology & botanydescription
The tonoplast is usually characterized by the presence of two electrogenic proton pumps: a vacuolartype H+-ATPase and a pyrophosphatase, as well as a putative water-channel-forming protein (γ-TIP). Using a post-embedding immunogold labelling technique, we have detected the presence of these transport-protein complexes not only in the tonoplast, but also in the plasma membrane and trans Golgi elements of maturing pea (Pisum sativum L.) cotyledons. These ultrastructural observations are supported by Western blotting with highly purified plasma-membrane fractions. In contrast to the vacuolar-type H+-ATPase, whose activity was not measurable, considerable pyrophosphatase activity was detected in the plasma-membrane fraction. These results are discussed in terms of a possible temporary repository for tonoplast proteins en route to the vacuole.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-01-01 | Planta |