6533b82bfe1ef96bd128e03b

RESEARCH PRODUCT

A method for screening diacetyl and acetoin-producing bacteria on agar plates

P. SchmittCharles DivièsCharles DivièsV. Phalip

subject

Agar platechemistry.chemical_compoundChromatographychemistrybiologyBiochemistryAcetoinGeneral Medicinebiology.organism_classificationApplied Microbiology and BiotechnologySolid mediumDiacetylBacteria

description

A simple method for screening bacteria for diacetyl and acetoin production was developed. This method is based on the ability of diacetyl and acetoin to form a red insoluble complex with α-naphthol in the presence of creatine. Addition of carboxymethyl-cellulose containing calcium citrate in the medium allowed discrimination between citrate utilizing and non-utilizing bacteria.

https://doi.org/10.1002/jobm.3620340412