Rapid and Standardized Quantitation of Hemolytic Activity of the Fourth Component of Human Complement

6533b82bfe1ef96bd128e1da

RESEARCH PRODUCT

Rapid and Standardized Quantitation of Hemolytic Activity of the Fourth Component of Human Complement

Christian Rittner Thomas Kaufmann Hans-joachim Kytzia

subject

Reproducibility Erythrocytes Polymorphism Genetic Chromatography Titration curve Chemistry Immunology C4A Complement C4 Hematology In Vitro Techniques Hemolysis Complement (complexity)Kinetics Humans Complement Pathway Classical

description

Based on a method that uses the fourth component of complement (C4)-deficient guinea pig serum to quantify the hemolytic activity of C4, we developed an assay that allows the processing of a large number of individual samples with high reproducibility. In contrast to the conventional procedure using titration curves of each sample to be determined, we can show that a single appropriate dilution of the sample allows accurate quantitation of hemolytic activity. The reliability of the procedure is demonstrated using either C4A- or C4B- deficient and normal individual samples.

year	journal	country	edition	language
1990-01-01	Complement and Inflammation

https://doi.org/10.1159/000463162

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