6533b82bfe1ef96bd128e22c
RESEARCH PRODUCT
Retinoid dynamics in chicken eye during pre- and postnatal development.
De Luca GMaria Concetta GueliConcetta M.a. NicotraAnna Maria PintaudiAntonino BonoAlessandra Paganinisubject
animal structuresgenetic structuresmedicine.drug_classClinical BiochemistryRetinoic acidDehydrogenaseTretinoinChick EmbryoEyeAndrologychemistry.chemical_compoundRetinoidsmedicineAnimalsRetinoidVitamin AMolecular Biologychemistry.chemical_classificationRetinolCell BiologyGeneral MedicineMetabolismAlcohol OxidoreductasesEnzymechemistryBiochemistryAcyltransferaseembryonic structuresRetinaldehydeRetinaldehydeChickensdescription
Changes in the steady state level of retinols, retinaldehydes and retinyl esters in the trans and 11-cis forms and trans retinoic acid were measured in whole chicken eye during development from day 6 in ovo to day 3 post-hatch. These retinoids, quantified by different HPLC systems, were detected in this time sequence: trans-retinol and trans-retinyl esters in the first week in ovo, 11-cis-retinol in the second week. The highest level of 11-cis-retinaldehyde and 11-cis-retinyl esters was reached at the end of development in ovo; however, their levels increased further after hatching. The retinoic acid level decreased at the end of the first week, rising again at the end of the second week. The enzyme activities involved in the metabolism of these retinoids-acyl-CoA: retinol acyltransferase, trans-retinol dehydrogenase, 11-cis-retinol dehydrogenase, trans-retinyl ester hydrolase and trans: 11-cis-retinol isomerase were also estimated and they were detectable already in the first week of development in ovo. At day 6 of the biosynthesis of retinoic acid by the retinaldehyde dehydrogenase activity from retina cytosol was also shown.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-03-01 | Molecular and cellular biochemistry |