6533b82cfe1ef96bd128eaa2
RESEARCH PRODUCT
Isolation of lactic acid-forming bacteria from biogas plants.
Jelena BohnAytül Yüksel-dadakStefan DrögeHelmut Königsubject
0301 basic medicineSilageBioengineeringBacillusBiologyReal-Time Polymerase Chain ReactionApplied Microbiology and Biotechnology03 medical and health scienceschemistry.chemical_compoundClostridiumBiogasLactobacillusBotanyLactic AcidPediococcusClostridiumBacteriafood and beveragesStreptococcusGeneral MedicineBiodiversityPlantsbiology.organism_classificationLactic acidManureLactobacillus030104 developmental biologychemistryBiofuelsFermentationFermentationPediococcusMultiplex Polymerase Chain ReactionBacteriaBiotechnologydescription
Direct molecular approaches provide hints that lactic acid bacteria play an important role in the degradation process of organic material to methanogenetic substrates in biogas plants. However, their diversity in biogas fermenter samples has not been analyzed in detail yet. For that reason, five different biogas fermenters, which were fed mainly with maize silage and manure from cattle or pigs, were examined for the occurrence of lactic acid-forming bacteria. A total of 197 lactic acid-forming bacterial strains were isolated, which we assigned to 21 species, belonging to the genera Bacillus, Clostridium, Lactobacillus, Pediococcus, Streptococcus and Pseudoramibacter-related. A qualitative multiplex system and a real-time quantitative PCR could be developed for most isolates, realized by the selection of specific primers. Their role in biogas plants was discussed on the basis of the quantitative results and on physiological data of the isolates.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-02-01 | Journal of biotechnology |