6533b82cfe1ef96bd128eb83
RESEARCH PRODUCT
Identification and functional expression of HCx31.9, a novel gap junction gene
Thomas W. WhiteMiduturu SrinivasRoberto BruzzoneAngela Trovato-salinaroNatale BelluardoHarris RippsGiuseppa MudòDaniele F. Condorellisubject
Patch-Clamp TechniquesIn silicoMolecular Sequence DataClinical BiochemistryXenopuscloningGene ExpressionConnexinConnexinsCell Linegap junctionMiceXenopus laevisGene expressionmedicineAnimalsHumansTissue DistributionAmino Acid SequenceCloning MolecularGenePhylogenybiologycloning; CNS; gap junctionGap junctionGap JunctionsCell BiologyGeneral MedicineTransfectionbiology.organism_classificationMolecular biologymedicine.anatomical_structureCerebral cortexOocytesCNSSequence Alignmentdescription
By combining in silico and bench molecular biology methods we have identified a novel human gap junction gene that encodes a protein designated HCx31.9. We have determined its human chromosomal location and gene structure, and we have identified a putative mouse ortholog, mCx30.2. We have observed the presence of HCx31.9 in human cerebral cortex, liver, heart, spleen, lung, and kidney and the presence of mCx30.2 in mouse cerebral cortex, liver and lung. Moreover, preliminary data on the electrophysiological properties of HCx31.9 have been obtained by functional expression in paired Xenopus oocytes and in transfected N2A cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-06-18 |