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RESEARCH PRODUCT
Puma (Puma concolor) epididymal sperm morphometry
Hernán CuchoCarles SolerAydee MezaEnrique AmpueroV. AlarconCésar Ordóñezsubject
Maleprincipal component analysisGermplasm BankUrologySemenEpididymal spermInvited Original ArticleSemen analysislcsh:RC870-923sperm morphometryAndrology03 medical and health sciences0302 clinical medicinePumaImage Processing Computer-AssistedmedicineAnimalsCell Shapeprincipal component analysis; puma; sperm morphometry; subpopulation; wild animalsubpopulationEpididymis030219 obstetrics & reproductive medicinemedicine.diagnostic_testbiologypuma0402 animal and dairy science04 agricultural and veterinary sciencesGeneral Medicinelcsh:Diseases of the genitourinary system. Urologybiology.organism_classificationSpermatozoa040201 dairy & animal scienceSpermwild animalSemen AnalysisSperm MotilitySperm morphologySperm HeadAcrosomedescription
The Andean puma (Puma concolor) has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13 morphometric parameters. The principal component (PC) analysis was performed followed by cluster analysis for the establishment of subpopulations. Two PC components were obtained, the first related to size and the second to shape. Three subpopulations were observed, corresponding to elongated and intermediate-size sperm heads and acrosomes, to large heads with large acrosomes, and to small heads with short acrosomes. In conclusion, puma spermatozoa showed no uniform sperm morphology but three clear subpopulations. These results should be used for future work in the establishment of an adequate germplasm bank of this species.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-09-01 | Asian Journal of Andrology |