6533b82cfe1ef96bd128f133

RESEARCH PRODUCT

Contribution to the research of microbial and RedOx bio-indicators for the early characterisation of wood diseases in grapevines

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Grapevine trunk diseases, linked to pathogenic fungi whose mechanism of action is still poorly characterised, are responsible for grapevine decline in vineyards throughout the world, causing significant losses in productivity and yield in the wine sector. Given the current lack of effective solutions to stop the establishment of the disease and the development of symptoms, which are irreversible but often late in relation to the pathogenic infection, early diagnosis of these diseases is essential. Considering the grapevine holobiont, i.e. the grapevine and the microbiota interacting with, we hypothesise that early bio-indicators of diseases would make it possible to identify a disturbance in homeostasis leading to a functional imbalance pre-existing the appearance of symptoms. Here, these potential bio-indicators would be linked to (i) the dysfunction of RedOx metabolism and (ii) changes in the microbial composition (bacteria, fungi) of the grapevine holobiont microbiota. Thus, in this study, grapevine samples (leaves, shoots, grafted wood and roots) as well as soil samples were collected in experiments conducted: (i) under controlled conditions via an infection model with one of the pathogens responsible for Botryosphaeria dieback, (ii) in the vineyard on grapevines affected by Esca. Analysis of the results shows that the microbial and RedOx metabolism markers we tested are, as they stand, difficult to use and probably unsuitable for early detection of the grapevine trunk diseases studied.