Analysis of fluorescent MRI contrast agent behavior in the liver and thoracic aorta of mice.

6533b82cfe1ef96bd128f14e

RESEARCH PRODUCT

Analysis of fluorescent MRI contrast agent behavior in the liver and thoracic aorta of mice.

E. Kahn G. Lizard D. Dumas Frédérique Frouin F. Menetrier J. F. Stoltz A. Todd-pokropek

subject

[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging Contrast Media Aorta Thoracic Mice MESH : Image Cytometry MESH: Microscopy Confocal MESH : Female MESH : Fluorescent Dyes MESH: Animals MESH : Algorithms [ SDV.IB.IMA ] Life Sciences [q-bio]/Bioengineering/Imaging health care economics and organizations Image Cytometry Mice Inbred BALB C Microscopy Confocal MESH: Fluorescent Dyes MESH: Staining and Labeling Liver MESH : Meglumine Female MESH : Organometallic Compounds Algorithms MESH: Aorta Thoracic education MESH: Mice Inbred BALB C MESH: Algorithms MESH: Meglumine MESH : Staining and Labeling Meglumine MESH: Contrast Media MESH : Mice Organometallic Compounds Animals MESH : Microscopy Confocal MESH: Mice MESH : Mice Inbred BALB C Fluorescent Dyes MESH : Aorta Thoracic MESH : Contrast Media Staining and Labeling MESH : Liver MESH: Organometallic Compounds MESH : Xanthenes [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging Xanthenes MESH: Xanthenes MESH : Animals MESH: Female MESH: Image Cytometry MESH: Liver

description

To characterize the behavior of magnetofluorescent products injected in mice intravenously.The magnetic resonance imaging (MRI) products were labelled with fluorescent molecules to examine the biodistribution process in vivo and observe them at the cellular level by means of confocal microscopy. Three-dimensional (3D) sequences of images were obtained by spectral analysis of sample preparations in a multiphoton confocal microscope and analyzed by the factor analysis of medical image sequence algorithm, which provides factor curves. Factor images are the result of image-processing methods that utilize information from emission spectra. Preparations are also screened in the counting mode to provide fluorescent lifetime imaging microscopy (FLIM) characterizations.Factor images and FLIM images can help to analyze MRI targeting inside the liver and thoracic aorta of mice. They show positive detection of Fe-Texas red and BOPTA-Eu in the liver and positive detection of Fe-Texas red and negative detection of BOPTA-Eu inside the thoracic aorta.This investigation established the utility of fluorescent MRI contrast agents as in vivo staining tools for cellular sites.

year	journal	country	edition	language
2004-08-01

https://hal.archives-ouvertes.fr/hal-00559067