6533b82cfe1ef96bd128f703

RESEARCH PRODUCT

Solid-phase extraction based on ground methacrylate monolith modified with gold nanoparticles for isolation of proteins

José Manuel Herrero-martínezMaría Jesús Lerma-garcíaErnesto F. Simó-alfonsoMaría Vergara-barberán

subject

SorbentMetal Nanoparticles02 engineering and technologyMethacrylate01 natural sciencesBiochemistryAnalytical ChemistryEnvironmental ChemistrySolid phase extractionMonolithBovine serum albuminSpectroscopygeographyChromatographygeography.geographical_feature_categorybiologyChemistryCytochrome cSolid Phase Extraction010401 analytical chemistryExtraction (chemistry)Proteins021001 nanoscience & nanotechnology0104 chemical sciencesColloidal goldMicroscopy Electron Scanningbiology.proteinMethacrylatesElectrophoresis Polyacrylamide GelGold0210 nano-technology

description

In this study, a novel polymeric material functionalized with gold nanoparticles (AuNPs) was prepared as solid-phase extraction (SPE) sorbent for isolation of proteins. The sorbent was synthesized from a powdered poly(glycidyl-co-ethylene dimethacrylate) monolith, and modified with ammonia, followed by immobilization of AuNPs on the pore surface of the material. To evaluate the performance of this SPE support, proteins were selected as test solutes, being the extraction conditions and other parameters (loading capacity and regenerative ability of sorbent) established. The results indicated that this sorbent could be employed to selectively capture proteins according to their pI, on the basis of the strong affinity of these biomacromolecules towards to AuNPs surface. The applicability of this sorbent was demonstrated by isolating protein species of interest (bovine serum albumin, cytochrome c and lectins in European mistletoe leaves), followed by SDS-PAGE analysis.

yearjournalcountryeditionlanguage
2015-12-12Analytica Chimica Acta
https://doi.org/10.1016/j.aca.2016.02.043