6533b82cfe1ef96bd128f78b

RESEARCH PRODUCT

Rapid adaptation of signaling networks in the fungal pathogen Magnaporthe oryzae

Stefan JacobLuis AnteloKarsten AndresenAlexander YemelinStefan BohnertChristiane Grünewald

subject

GlycerolMagnaportheved/biology.organism_classification_rank.speciesMutantGenomeSalt StressTranscriptome0302 clinical medicineOsmoregulationLoss of Function MutationGene Expression Regulation FungalGene Regulatory NetworksSuppressorReestablishment of osmoregulation0303 health sciencesbiologyMagnaporthe oryzaeRewiringAdaptation PhysiologicalRapid adaptationCell biologyMagnaportheOsmoregulationEpigeneticsGenome FungalBiotechnologySignal TransductionResearch Articlelcsh:QH426-470lcsh:BiotechnologyDioxolesFungal Proteins03 medical and health sciencesDrug Resistance Fungallcsh:TP248.13-248.65GeneticsPyrrolesModel organismGene030304 developmental biologyPlant DiseasesOsmotic concentrationved/biologyGene Expression ProfilingEvolution of signaling networksHOG pathwayOryzabiology.organism_classificationlcsh:Genetics030217 neurology & neurosurgery

description

Abstract Background One fundamental question in biology is how the evolution of eukaryotic signaling networks has taken place. “Loss of function” (lof) mutants from components of the high osmolarity glycerol (HOG) signaling pathway in the filamentous fungus Magnaporthe oryzae are viable, but impaired in osmoregulation. Results After long-term cultivation upon high osmolarity, stable individuals with reestablished osmoregulation capacity arise independently from each of the mutants with inactivated HOG pathway. This phenomenon is extremely reproducible and occurs only in osmosensitive mutants related to the HOG pathway – not in other osmosensitive Magnaporthe mutants. The major compatible solute produced by these adapted strains to cope with high osmolarity is glycerol, whereas it is arabitol in the wildtype strain. Genome and transcriptome analysis resulted in candidate genes related to glycerol metabolism, perhaps responsible for an epigenetic induced reestablishment of osmoregulation, since these genes do not show structural variations within the coding or promotor sequences. Conclusion This is the first report of a stable adaptation in eukaryotes by producing different metabolites and opens a door for the scientific community since the HOG pathway is worked on intensively in many eukaryotic model organisms.

yearjournalcountryeditionlanguage
2019-09-30
https://doi.org/10.21203/rs.2.9870/v2