6533b82cfe1ef96bd128fe13

RESEARCH PRODUCT

Simultaneous gas chromatographic determination of aliphatic C2−C20 n-carboxylic acids and their methyl esters using SE-30 and OV-351 quartz capillary columns

I. O. O. Korhonen

subject

chemistry.chemical_classificationChromatographyFree acidChemistryCapillary actionElutionCarboxylic acidOrganic ChemistryClinical BiochemistryBiochemistryComplete resolutionAnalytical ChemistryMethyl octanoateAcetic acidchemistry.chemical_compoundQuartz

description

Simultaneous determination of aliphatic C2−C20 carboxylic acids and their methyl esters was carried out using SE-30 and OV-351 quartz capillary columns with temperature programming. Complete resolution of all 36 components was achieved. On SE-30 the methyl ester and the corresponding free acid are eluted in turn, whereas on OV-351 acetic acid eluted after methyl octanoate, after which the Cn methyl ester and Cn-6 carboxylic acid (n>9) are eluted one after the other. A non-polar SE-30 column is better for long-chain acids giving sharp peaks without tailing, the analysis time of the mixture being less than 16 minutes. The weight response correction factors for compounds under optimum operating conditions are given.

yearjournalcountryeditionlanguage
1983-02-01Chromatographia
https://doi.org/10.1007/bf02259342