Lemon Oils Attenuate the Pathogenicity of Pseudomonas aeruginosa by Quorum Sensing Inhibition

6533b82cfe1ef96bd129012c

RESEARCH PRODUCT

Lemon Oils Attenuate the Pathogenicity of Pseudomonas aeruginosa by Quorum Sensing Inhibition

Elena Cartagena Elena Cartagena María Rosa Alberto María Rosa Alberto María Constanza Luciardi María Amparo Blázquez Mario Eduardo Arena Mario Eduardo Arena

subject

Citrus CITRUS PEEL Pharmaceutical Science Swarming motility pyocyanin medicine.disease_cause biofilm Analytical Chemistry law.invention //purl.org/becyt/ford/1 [https]Terpene chemistry.chemical_compound QD241-441 law Drug Resistance Multiple Bacterial QUORUM SENSING Drug Discovery Citrus peel Food science Bicyclic Monoterpenes 0303 health sciences Pancreatic Elastase Virulence quorum sensing 04 agricultural and veterinary sciences 040401 food science Anti-Bacterial Agents motility Chemistry (miscellaneous)Pseudomonas aeruginosa Molecular Medicine BIOFILM VIRULENCE FACTORS Signal Transduction PYOCYANIN Virulence Factors Cyclohexane Monoterpenes <i>Citrus</i> peel Article Gas Chromatography-Mass Spectrometry 03 medical and health sciences 0404 agricultural biotechnology Pyocyanin Bacterial Proteins MOTILITY Oils Volatile medicine Plant Oils elastase Physical and Theoretical Chemistry //purl.org/becyt/ford/1.6 [https]Essential oil Volatile Organic Compounds Limonene 030306 microbiology Pseudomonas aeruginosa Organic Chemistry Biofilm Quorum sensing chemistry Biofilms Pyocyanine Limonene ELASTASE

description

The chemical composition of three Citrus limon oils: lemon essential oil (LEO), lemon terpenes (LT) and lemon essence (LE), and their influence in the virulence factors production and motility (swarming and swimming) of two Pseudomonas aeruginosa strains (ATCC 27853 and a multidrug-resistant HT5) were investigated. The main compound, limonene, was also tested in biological assays. Eighty-four compounds, accounting for a relative peak area of 99.23%, 98.58% and 99.64%, were identified by GC/MS. Limonene (59–60%), γ-terpinene (10–11%) and β-pinene (7–15%) were the main compounds. All lemon oils inhibited specific biofilm production and bacterial metabolic activities into biofilm in a dose-dependent manner (20–65%, in the range of 0.1–4 mg mL−1) of both strains. Besides, all samples inhibited about 50% of the elastase activity at 0.1 mg mL−1. Pyocyanin biosynthesis decreases until 64% (0.1–4 mg mL−1) for both strains. Swarming motility of P. aeruginosa ATCC 27853 was completely inhibited by 2 mg mL−1 of lemon oils. Furthermore, a decrease (29–55%, 0.1–4 mg mL−1) in the synthesis of Quorum sensing (QS) signals was observed. The oils showed higher biological activities than limonene. Hence, their ability to control the biofilm of P. aeruginosa and reduce the production of virulence factors regulated by QS makes lemon oils good candidates to be applied as preservatives in the food processing industry. Fil: Luciardi, María Constanza. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Blázquez, María Amparo. Universidad de Valencia; España Fil: Alberto, Maria Rosa. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Cartagena, Elena. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina

year	journal	country	edition	language
2021-05-01	Molecules

https://doi.org/10.3390/molecules26102863