6533b82dfe1ef96bd12908ec

RESEARCH PRODUCT

Applications of stable V79-derived cell lines expressing rat cytochromes P4501A1, 1A2, and 2B1.

C. DoehmerFranz OeschHansruedi GlattJohannes DoehmerCatherine WölfelS. DograKarl-ludwig PlattAlbrecht Seidel

subject

CytochromeHealth Toxicology and Mutagenesis78-Dihydro-78-dihydroxybenzo(a)pyrene 910-oxideGenetic VectorsDNA RecombinantHamsterHydroxylationToxicologyBiochemistryChinese hamsterlaw.inventionCell LineDihydroxydihydrobenzopyrenesMixed Function OxygenasesHydroxylationchemistry.chemical_compoundCricetulusCytochrome P-450 Enzyme SystemlawCytochrome P-450 CYP1A2CricetinaeBenzo(a)pyreneAnimalsCloning MolecularCytotoxicityCyclophosphamideBiotransformationPharmacologybiologyCytochrome P450General Medicinebiology.organism_classificationMolecular biologyRatsBiochemistrychemistryCell cultureRecombinant DNAbiology.proteinOxidoreductases

description

1. Chinese hamster V79-derived cell lines, stably expressing cytochromes P4501A1, 1A2, and 2B1 activities, were constructed by genetic engineering in continuation of our work to establish a battery of V79 derived cell lines designed to study the metabolism of xenobiotics. 2. Cell lines XEM1 and XEM2, expressing cytochrome P4501A1, were capable of the O-dealkylation of 7-ethoxycoumarin and the hydroxylation of benzo[a]pyrene. 3. Cell lines XEMd.MZ and XEMd.NH, expressing P4501A2, were shown to hydroxylate 17 beta-estradiol and 2-aminofluorene. 4. Cell line SD1, expressing cytochrome P4502B1, was able to hydroxylate testosterone stereo- and regio-specifically at the 16 alpha and 16 beta positions. 5. Cell lines were validated in mutagenicity, cytotoxicity, and metabolism studies employing benzo[a]pyrene, trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene, cyclophosphamide, ifosfamide, and picene. 6. Construction of metabolically-competent V79-derived cell lines be recombinant DNA technology will be a fundamental improvement for the evaluation of the cytotoxic, genotoxic and pharmacological properties of a chemical.

yearjournalcountryeditionlanguage
1992-09-01Xenobiotica; the fate of foreign compounds in biological systems
10.3109/00498259209051863https://pubmed.ncbi.nlm.nih.gov/1441600