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RESEARCH PRODUCT
Sensitive Monoclonal Antibody-Based Immunoassays for Kresoxim-methyl Analysis in QuEChERS-Based Food Extracts
Josep V. MercaderRosario López-morenoAntonio Abad-fuentesConsuelo AgullóFrancesc A. Esteve-turrillasAntonio Abad-somovillasubject
QuEChERSmedicine.drug_classEnzyme-Linked Immunosorbent AssayFood ContaminationRapid methodsQuechersMass spectrometryMonoclonal antibodyFragariaDeming regressionSolanum lycopersicummedicinePhenylacetatesDetection limitResidue (complex analysis)ChromatographyChemistrySolid Phase ExtractionPesticide ResiduesAntibodies MonoclonalResiduesGeneral ChemistryStrobilurinsFungicides IndustrialCompetitive ELISAFruitStrobilurinMethacrylatesGas chromatographyCucumis sativusGeneral Agricultural and Biological SciencesKresoxim-methyldescription
Kresoxim-methyl is nowadays widely used to combat a diversity of common diseases affecting high-value crops. In this article, we report the development and characterization of two novel immunoassays for the analysis of this pioneer strobilurin fungicide, and for the first time, a validation study with food samples was performed. A direct and an indirect competitive immunoassay based on a new anti-kresoxim-methyl monoclonal antibody were developed for sensitive and specific chemical analysis. Optimized assays showed limits of detection of 0.1 μg/L. Fruit and vegetable samples were extracted with acetonitrile by the QuEChERS procedure and analyzed by the developed immunoassays after a simple dilution in buffer, affording limits of quantification below US and European maximum residue limits. Immunochemical results of samples from kresoxim-methyl-sprayed strawberry fields demonstrated good statistical agreement with gas chromatography coupled to mass spectrometry as reference technique. © 2014 American Chemical Society.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-01-01 | Journal of Agricultural and Food Chemistry |