6533b82dfe1ef96bd1291179
RESEARCH PRODUCT
Multipumping Nitrite Determination in Exhaled Breath Condensate
ÁNgel Morales-rubioMiguel De La GuardiaAntonio Peláez‐hernándezCelia Morales‐rubioEva Ródenas-torralbasubject
Flow injection analysisDetection limitChromatographyChemistryCoefficient of variationAtomic and Molecular Physics and OpticsAnalytical Chemistrychemistry.chemical_compoundGriess testReagentExhaled breath condensateNitritePhosphoric acidSpectroscopydescription
Abstract A flow system based on multicommutation is proposed for the rapid, clean, and inexpensive determination of nitrites in small volumes of breath condensates. The procedure exploits the colorimetric detection of nitrite with the Griess reagent [0.03% naphthylethylene diamine dihydrochloride (NED), 0.5% sulpfhanilamide, and 3.0% phosphoric acid] in acidic medium at 540 nm correcting the variations of the baseline with measurements at 424 nm. The flow system was designed with a set of solenoid micropumps to minimize sample and reagent consumption and waste generation. The detection limit was estimated as 3.8 ng mL−1 (99.7% confidence level) with a linear response ranging up to 500 ng mL−1. The coefficient of variation was estimated as 0.7% for a solution containing 300 ng mL−1 nitrite (n=9). Approximately 144 determinations can be carried out per hour, consuming only 678.4 µg Griess reagent and generating 1.184 mL of effluent per determination, thus providing an environmentally friendly alternative an...
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-12-01 | Spectroscopy Letters |