6533b82dfe1ef96bd1291351

RESEARCH PRODUCT

Additional file 1 of Comparison of time and dose dependent gene expression and affected pathways in primary human fibroblasts after exposure to ionizing radiation

subject

description

Additional file 1: Web Figure 1. Representative measurements of the cell cycle distribution of HOECHST33258-stained fibroblasts by flow cytometry during (A) log-phase growth or (B) after G0/1 synchronization over 14 days for radiation experiments. Web Figure 2. Total number of differentially expressed genes in human fibroblasts from cancer free-controls at 0.25 h, 2 h and 24 h after exposure to low (0.05 Gray (Gy)) or high dose (2Gy) of X-rays compared to unirradiated fibroblasts (N = 3). Web Figure 3. Correlation of RNA quality metrics (RIN, Qbit RNA-concentration), expression variation (PC1–3) and number of aligned reads (aligned reads, aligned reads normalized) for all experiments. The color indicates the sequencing run (red = run 1, blue = run 2). Web Figure 4. Relative expression of Cyclin-Dependent Kinase Inhibitor 1A (CDKN1A) in Real-Time Quantitative Polymerase-Chain-Reaction (qPCR) analyzing the expression of CDKN1A in fibroblasts of 6 participants 2 h and 4 h after exposure to 0.05 Gray (Gy) or 2Gy ionizing radiation compared to sham-irradiated samples (0Gy, reference). *** p < 0.001. Web Figure 5. Relative expression of Mouse double minute 2 homolog (MDM2) in Real-Time Quantitative Polymerase-Chain-Reaction (qPCR) analyzing the expression of MDM2 in fibroblasts of 6 participants 2 h and 4 h after exposure to 0.05 Gray (Gy) or 2Gy ionizing radiation compared to sham-irradiated samples (0Gy, reference). *** p < 0.001. Web Figure 6. Expression variation in fibroblasts summarized for all experiments and attributed to time point post irradiation (circle = 2 h, cross = 4 h) and dose (orange = 0 Gray (Gy), blue = 0.05Gy, green = 2Gy).