6533b82dfe1ef96bd129141c

RESEARCH PRODUCT

Medium for Screening Leuconostoc oenos Strains Defective in Malolactic Fermentation

Charles DivièsJ. LinP. SchmittJean-françois CavinHervé Prévost

subject

Bromocresol greenEcologybiologyfood and beveragesFructosebiology.organism_classificationStreptococcaceaeApplied Microbiology and Biotechnologychemistry.chemical_compoundchemistryBiochemistrypH indicatorMalolactic fermentationMethodsLeuconostocCelluloseBacteriaFood ScienceBiotechnology

description

A new sensitive medium was developed to screen and isolate mutagenic Leuconostoc oenos strains defective in malolactic fermentation. The essential components of the medium included fructose (22 mM), l -malic acid (74.6 mM), bromocresol green (as pH indicator), and cellulose powder. The wild-type colonies turned blue, but defective malolactic colonies gave an acid reaction and remained yellow-green.

https://europepmc.org/articles/PMC184191/