6533b82dfe1ef96bd129142e
RESEARCH PRODUCT
Axial gain resolution in optical sectioning fluorescence microscopy by shaded-ring filters.
C. Ibáñez-lópezM. T. CaballeroManuel Martínez-corralGenaro Saavedrasubject
Materials scienceMicroscopegenetic structuresOptical sectioningbusiness.industryResolution (electron density)eye diseasesAtomic and Molecular Physics and Opticslaw.inventionOpticsConfocal microscopylawLight sheet fluorescence microscopyElectric fieldFluorescence microscopesense organsbusinessBiological imagingdescription
We present a new family of pupil masks to control the axial component of the intensity distribution in the focal region of tightly focused light fields. The filters, which consist of a circular clear pupil with a single shaded ring, allow to control the width of the central lobe of the axial spot together with the residual sidelobes energy. The filters can be applied to improve the optical sectioning capacity of different scanning microscopes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-05-26 | Optics express |