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RESEARCH PRODUCT
Deciliation: A stressful event for Paracentrotus lividus embryos.
Fabrizio GianguzzaMaria Carmela RoccheriKaroly OnoratoD. CascinoCaterina Casanosubject
CytoplasmEmbryo NonmammalianBiophysicsBiochemistryParacentrotus lividusFight-or-flight responseMethionineStress Physiologicalbiology.animalProtein biosynthesisAnimalsRegenerationElectrophoresis Gel Two-DimensionalCiliaHeat shockMolecular BiologySea urchinCell NucleusSaline Solution HypertonicbiologyProteinsEmbryoCell BiologyGastrulaSea urchin embryobiology.organism_classificationMolecular biologyCell biologyProtein BiosynthesisSea UrchinsElectrophoresis Polyacrylamide Geldescription
In this report, by using mono- and two-dimensional electrophoretic analysis, we demonstrate that deciliation on sea urchin embryos induces a stress response. Deciliation indeed causes not only the activation of ciliary subroutine, but also a transient decrease of bulk protein synthesis. This decrease is in agreement with our previous results on heat shock response in sea urchin, although deciliation does not induce the expression of the same main hsp set. We were able to characterize one main deciliation-stress protein of 40 kDa whose expression is transiently induced by deciliation and whose localisation is likely to be nuclear.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-08-15 | Biochemical and biophysical research communications |