6533b82dfe1ef96bd12915c4
RESEARCH PRODUCT
Low-Cost Synthesis of Smart Biocompatible Graphene Oxide Reduced Species by Means of GFP.
Angela CuttittaNerina ArmataFabrizio Lo CelsoFlavio PendolinoSalvatore MazzolaTiziana MasulloPaolo Colombosubject
Absorption (pharmacology)HemolysiPhotoluminescenceMaterials scienceBiocompatibilityGreen Fluorescent ProteinsOxideNanotechnologyGraphite oxideBioengineeringBiocompatible Materials02 engineering and technology010402 general chemistryGFP01 natural sciencesBiochemistryApplied Microbiology and Biotechnologylaw.inventionchemistry.chemical_compoundlawGraphiteMolecular BiologyGraphene oxideReductionGraphenegraphene oxide; graphite oxide; GFP; reduction; biocompatibility; hemolysisOxidesGeneral Medicine021001 nanoscience & nanotechnology0104 chemical scienceschemistryChemical engineeringDrug deliveryGraphite oxideBiocompatibilityGraphite0210 nano-technologyBiotechnologydescription
The aim of this work is focused on the engineering of biocompatible complex systems composed of an inorganic and bio part. Graphene oxide (GO) and/or graphite oxide (GtO) were taken into account as potential substrates to the linkage of the protein such as Anemonia sulcata recombinant green fluorescent protein (rAsGFP). The complex system is obtained through a reduction process between GO/GtO and rAsGFP archiving an environmentally friendly biosynthesis. Spectroscopic measurements support the formation of reduced species. In particular, photoluminescence shows a change in the activity of the protein when a bond is formed, highlighted by a loss of the maximum emission signal of rAsGFP and a redshift of the maximum absorption peak of the GO/GtO species. Moreover, the hemolysis assay reveals a lower value in the presence of less oxidized graphene species providing evidence for a biocompatible material. This singular aspect can be approached as a promising method for circulating pharmaceutical preparations via intravenous administration in the field of drug delivery.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-01-01 | Applied biochemistry and biotechnology |