Super-high-speed liquid chromatography of proteins and peptides on non-porous Micra NPS-RP packings

6533b82dfe1ef96bd1291baa

RESEARCH PRODUCT

Super-high-speed liquid chromatography of proteins and peptides on non-porous Micra NPS-RP packings

A Kourganov K.k. Unger T. Issaeva

subject

chemistry.chemical_classification Peptide fragment Chromatography biology Elution Chemistry Organic Chemistry Analytical chemistry Chymotrypsinogen Peptide General Medicine Reversed-phase chromatography Ovotransferrin Biochemistry High-performance liquid chromatography Analytical Chemistry biology.protein Porosity

description

Abstract The new generation of non-porous silica RP packings commercially available from Micra Scientific was tested for separations of peptides and proteins by means of the gradient HPLC. Extremely high-speed separations were achieved using conventional chromatographic equipment: six proteins could be completely separated within six seconds. Tryptic digest peptides could be resolved in more then 40 components within 2–3 min. The effect of the experimental parameters such as temperature, flow rate etc. was investigated.

year	journal	country	edition	language
1999-06-01	Journal of Chromatography A

https://doi.org/10.1016/s0021-9673(99)00360-x

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