0000000000542170
AUTHOR
T. Issaeva
showing 2 related works from this author
Packings of an unidimensional regular pore structure as model packings in size-exclusion and inverse size-exclusion chromatography
1996
Using porous aluminas and aluminosilicates with a regular and well-defined pore structure an attempt was made to correlate the SEC data of polystyrenes with established theoretical models. The pore structure of the two types of packings was extensively characterized by means of transmission and scanning electron microscopy, nitrogen sorption and mercury porosimetry. The SEC distribution coefficients measured experimentally were correlated with the distribution coefficients calculated from the theoretical models for flexible polymers. A good correlation was observed for the packing of the simplest pore morphology, but remarkable deviations appeared for the materials with a more complex pore …
Super-high-speed liquid chromatography of proteins and peptides on non-porous Micra NPS-RP packings
1999
Abstract The new generation of non-porous silica RP packings commercially available from Micra Scientific was tested for separations of peptides and proteins by means of the gradient HPLC. Extremely high-speed separations were achieved using conventional chromatographic equipment: six proteins could be completely separated within six seconds. Tryptic digest peptides could be resolved in more then 40 components within 2–3 min. The effect of the experimental parameters such as temperature, flow rate etc. was investigated.