6533b82dfe1ef96bd1291c86
RESEARCH PRODUCT
Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d
Daniel HessMiguel A. Andrade-navarroIrmgard U. HaussmannDominik JacobJean-yves RoignantMarco BiggiogeraIrene MasielloTina LenceMark HelmRodrigo VillaseñorRodrigo VillaseñorPhilip KnucklesNastasja KreimMarc BühlerMarc BühlerTina HaresMatthias SollerSarah H. CarlSarah H. Carlsubject
0301 basic medicineZinc fingerMethyltransferase complexMRNA modificationRNA-binding proteinMethylationBiologyDNA-binding proteinCell biology03 medical and health sciences030104 developmental biologyFLACC scaleGeneticsDrosophila ProteinDevelopmental Biologydescription
N6-methyladenosine (m6A) is the most abundant mRNA modification in eukaryotes, playing crucial roles in multiple biological processes. m6A is catalyzed by the activity of methyltransferase-like 3 (Mettl3), which depends on additional proteins whose precise functions remain poorly understood. Here we identified Zc3h13 (zinc finger CCCH domain-containing protein 13)/Flacc [Fl(2)d-associated complex component] as a novel interactor of m6A methyltransferase complex components in Drosophila and mice. Like other components of this complex, Flacc controls m6A levels and is involved in sex determination in Drosophila. We demonstrate that Flacc promotes m6A deposition by bridging Fl(2)d to the mRNA-binding factor Nito. Altogether, our work advances the molecular understanding of conservation and regulation of the m6A machinery.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-03-01 | Genes & Development |