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RESEARCH PRODUCT
Active and Secretory IgA-Coated Bacterial Fractions Elucidate Dysbiosis in Clostridium difficile Infection
Mária DážunkovážáAndrážáés MoyaJorge F. Vážáéázquez-castellanosAlejandro ArtachoXinhua ChenCiaran KellyGiuseppe D’auriaHideyuki Tamakisubject
0301 basic medicineClostridium Cluster IVmedicine.drug_class030106 microbiologyAntibioticslcsh:QR1-502Microbiologylcsh:MicrobiologyantibioticsMicrobiologyHost-Microbe Biology03 medical and health sciencesClostridium difficile infectionmedicineMicrobiomeMolecular Biology16S rRNA gene sequencinghuman gut microbiomebiologyLactobacillalesdysbiosisClostridium difficilebiology.organism_classificationmedicine.diseaseQR1-502030104 developmental biologyBayesian networksFusobacteriumImmunologysecretory immunoglobulin ADysbiosisBacteriafluorescence-activated cell sortingResearch Articledescription
C. difficile is a major enteric pathogen with worldwide distribution. Its expansion is associated with broad-spectrum antibiotics which disturb the normal gut microbiome. In this study, the DNA sequencing of highly active bacteria and bacteria opsonized by intestinal secretory immunoglobulin A (SIgA) separated from the whole bacterial community by FACS elucidated how the gut dysbiosis promotes C. difficile infection (CDI). Bacterial groups with inhibitory effects on C. difficile growth, such as Lactobacillales, were mostly inactive in the CDI patients. C. difficile was typical for the bacterial fraction opsonized by SIgA in patients with CDI, while Fusobacterium was characteristic for the SIgA-opsonized fraction of the controls. The study demonstrates that sequencing of specific bacterial fractions provides additional information about dysbiotic processes in the gut. The detected patterns have been confirmed with the whole patient cohort independently of the taxonomic differences detected in the nonfractionated microbiomes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-05-01 | mSphere |