6533b82dfe1ef96bd1291f3f
RESEARCH PRODUCT
Mass spectrometry applications
Jordi MañesCarla SolerJosep Rubertsubject
Ion-mobility spectrometry–mass spectrometryLiquid chromatography–mass spectrometryChemistryLife ScienceComputational biologyDirect electron ionization liquid chromatography–mass spectrometry interfaceProteomicsMass spectrometryMozzarella cheeseMass spectrometry imagingdescription
The history of proteomics dates back to the discovery of two-dimensional gels in the 1970s, which provided the first feasible way of displaying hundreds or thousands of proteins on a single gel. Despite mass spectrometry being restricted for a long time to small and thermostable compounds, the development in the late 1980s of two techniques for the routine and general formation of molecular ions of intact biomolecules changed this situation and mass spectrometry has become an indispensable tool for proteomics research. The aim of this chapter is to review the major types of MS instruments used in proteomics analysis and to discuss strategies for the analysis of whole proteins and peptides obtained after degradation. Finally, major applications of mass spectrometry-based proteomics in food safety are summarized.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-11-30 |