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RESEARCH PRODUCT

Assessment of Toxic Effects of Ochratoxin A in Human Embryonic Stem Cells

Eva M. MateoSlaven ErcegMisericordia JiménezFrancisco Javier Rodriguez JimenezMa. Angeles Garcia-esparzaJosé Miguel SoriaMaria Amparo Perez AragoIvan Zipancic

subject

Ochratoxin AHealth Toxicology and MutagenesisCellHuman Embryonic Stem Cellslcsh:MedicineToxicologychemistry.chemical_compoundMicotoxinas.Stem cells.Cytotoxicityhuman Stem CellsCells Cultured0303 health sciencesOchratoxin A.030302 biochemistry & molecular biologyOchratoxinsCell biologymedicine.anatomical_structureTeratogensembryonic structurescytotoxicityStem cellCell SurvivalOcratoxina A.Food ContaminationBiologyModels BiologicalArticle03 medical and health sciencesCélulas madre.mycotoxinsmedicineCell AdhesionHumansMycotoxin030304 developmental biologyCell Proliferationcell cultureMycotoxins.lcsh:REmbriología humana.Embryonic stem cellEmbryology Human.In vitroOxidative StressBlastocystchemistryCell culturecellsOchratoxin A (OTA)

description

Ochratoxin A (OTA) is a mycotoxin produced by different Aspergillus and Penicillium species, and it is considered a common contaminant in food and animal feed worldwide. On the other hand, human embryonic stem cells (hESCs) have been suggested as a valuable model for evaluating drug embryotoxicity. In this study, we have evaluated potentially toxic effects of OTA in hESCs. By using in vitro culture techniques, specific cellular markers, and molecular biology procedures, we found that OTA produces mild cytotoxic effects in hESCs by inhibiting cell attachment, survival, and proliferation in a dose-dependent manner. Thus, we suggest that hESCs provide a valuable human and cellular model for toxicological studies regarding preimplantation stage of human fetal development.

yearjournalcountryeditionlanguage
2019-04-01Toxins
10.3390/toxins11040217http://dx.doi.org/10.3390/toxins11040217