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RESEARCH PRODUCT
Column technology in liquid chromatography
Egidijus MachtejevasKlaus K. UngerStefan Lamottesubject
ChromatographyMaterials sciencePeriod (periodic table)Resolution (mass spectrometry)010401 analytical chemistrySilica column02 engineering and technology021001 nanoscience & nanotechnology01 natural sciencesColumn (database)High-performance liquid chromatography0104 chemical sciencesHighly sensitiveAdsorptionSurface modification0210 nano-technologyPorosityColumn designdescription
This chapter deals with the most important part of the liquid chromatography (LC) system: the column enabling the efficient and fast resolution of complex mixtures. It is divided into seven sections under the overall aspects of manufacture, operation, and evaluation of analytical columns for the user. The first three sections highlight the column design and hardware, followed by a thorough treatment of the properties of microparticulate silica adsorbents as packing material, stationary phases performed by surface functionalization, and the column filling process. Then, the implementation of the column into the LC system is discussed, leading into chromatographic column testing as a C18-bonded reversed silica column and its maintenance. Section 3.7 is devoted to today’s column market, focusing on the three main types of silica columns: fully porous microparticulate silica columns, columns with core-shell silica particles, and monolithic silica columns. Within a period of 40 years, analytical high-performance LC (HPLC) has generated high-resolution, robust, and rugged reversed-phase silica columns, which enable efficient, highly sensitive, and fast separations of low-molecular–weight substances based on validated methods.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-01-01 |