Column technology in liquid chromatography

This chapter deals with the most important part of the liquid chromatography (LC) system: the column enabling the efficient and fast resolution of complex mixtures. It is divided into seven sections under the overall aspects of manufacture, operation, and evaluation of analytical columns for the user. The first three sections highlight the column design and hardware, followed by a thorough treatment of the properties of microparticulate silica adsorbents as packing material, stationary phases performed by surface functionalization, and the column filling process. Then, the implementation of the column into the LC system is discussed, leading into chromatographic column testing as a C18-bond…