6533b82efe1ef96bd1292665
RESEARCH PRODUCT
Analysis of Specific Protein-DNA Interactions
Nils RostoksIndrikis Muizniekssubject
Regulation of gene expressionCalf-intestinal alkaline phosphatasechemistry.chemical_compoundbiologyChemistryGene expressionbiology.proteinElectrophoretic mobility shift assayComputational biologyDNA condensationGenomeDNAChromatindescription
The central issue in the regulation of genome functions is the mechanism of sequence-specific protein-nucleic acid interactions. Gene expression, replication, recombination and DNA condensation in chromatin are steered by binding of regulatory protein ligands to specific sites in DNA. Numerous methods have been developed to study protein-DNA interactions. In this chapter we discuss two widely used and straightforward approaches to address this problem.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-01-01 |