6533b82efe1ef96bd12928ca

RESEARCH PRODUCT

Sequence of a sea urchin hsp70 gene and its 5' flanking region.

Maria Carmela RoccheriMarta Di CarloGiovanni GiudiceMaria La RosaGabriella Sconzo

subject

5' flanking regionMolecular Sequence DataRestriction MappingBiologyExonSequence Homology Nucleic AcidConsensus SequenceGeneticsConsensus sequenceAnimalsAmino Acid SequencePromoter Regions GeneticGenePeptide sequenceHeat-Shock ProteinsGeneticsBase SequenceStructural geneNucleic acid sequenceGeneral MedicineExonsMolecular biologyIntronsGenesRegulatory sequenceSea Urchins

description

We report the nucleotide sequence of a 4470-bp fragment derived from a sea urchin genomic clone containing part of a heat-shock protein 70 (Hsp70)-encoding gene. This fragment, named hsp70 gene II, contains 1271 bp of the flanking region and 3299 bp of structural gene sequence interrupted by five introns and encoding the N-terminal 371 amino acids (aa) of the protein. The 5' flanking region contains a putative TATA element, two CCAAT boxes, four heat-shock consensus sequence elements (hse) and one consensus sequence for binding of Sp1. Remarkable homologies were observed for deduced aa sequence and intron-exon organization between hsp70 gene II and rat hsc73 gene.

yearjournalcountryeditionlanguage
1990-12-01Gene
10.1016/0378-1119(90)90267-uhttps://pubmed.ncbi.nlm.nih.gov/2269441