6533b82efe1ef96bd1292914
RESEARCH PRODUCT
Effects of heat treatment on hydrogen production potential and microbial community of thermophilic compost enrichment cultures
Jukka RintalaJaakko A. PuhakkaMarika E. NissiläHanne P. Tähtisubject
Hot TemperatureEnvironmental EngineeringMicrobial metabolismBioengineeringPolymerase Chain ReactionEnrichment cultureMicrobiologySoilchemistry.chemical_compoundRNA Ribosomal 16SClostridiaceaeFood scienceClostridium stercorariumCelluloseCelluloseWaste Management and DisposalSoil MicrobiologyHydrogen productionBiological Oxygen Demand AnalysisBacteriabiologyDenaturing Gradient Gel ElectrophoresisRenewable Energy Sustainability and the EnvironmentGeneral MedicineDark fermentationbiology.organism_classification220 Industrial biotechnologychemistryBiofuelsFermentative hydrogen productionFermentationHydrogendescription
Cellulosic plant and waste materials are potential resources for fermentative hydrogen production. In this study, hydrogen producing, cellulolytic cultures were enriched from compost material at 52, 60 and 70°C. Highest cellulose degradation and highest H(2) yield were 57% and 1.4 mol-H(2) mol-hexose(-1) (2.4 mol-H(2) mol-hexose-degraded(-1)), respectively, obtained at 52°C with the heat-treated (80°C for 20 min) enrichment culture. Heat-treatments as well as the sequential enrichments decreased the diversity of microbial communities. The enrichments contained mainly bacteria from families Thermoanaerobacteriaceae and Clostridiaceae, from which a bacterium closely related to Thermoanaerobium thermosaccharolyticum was mainly responsible for hydrogen production and bacteria closely related to Clostridium cellulosi and Clostridium stercorarium were responsible for cellulose degradation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-03-01 |