Synthesis, X-ray Structure Determination, and Comprehensive Photochemical Characterization of (Trifluoromethyl)diazirine-Containing TRPML1 Ligands

6533b82efe1ef96bd1292a5a

RESEARCH PRODUCT

Synthesis, X-ray Structure Determination, and Comprehensive Photochemical Characterization of (Trifluoromethyl)diazirine-Containing TRPML1 Ligands

Simon Grabowsky Michał Andrzejewski Kevin Schwickert Tanja Schirmeister

subject

Trifluoromethyl Photoaffinity labeling TRPML 010405 organic chemistry X-Rays Organic Chemistry X-ray Photoaffinity Labels Ligands 010402 general chemistry Photochemistry Mass spectrometry 01 natural sciences Mass Spectrometry 0104 chemical sciences Characterization (materials science)chemistry.chemical_compound Diazomethane chemistry 540 Chemistry Diazirine 570 Life sciences; biology Ion channel

description

Potential (trifluoromethyl)diazirine-based TRPML1 ion channel ligands were designed and synthesized, and their structures were determined by single-crystal X-ray diffraction analysis. Photoactivation studies via 19F NMR spectroscopy and HPLC-MS analysis revealed distinct kinetical characteristics in selected solvents and favorable photochemical properties in an aqueous buffer. These photoactivatable TRPML activators represent useful and valuable tools for TRPML photoaffinity labeling combined with mass spectrometry.

year	journal	country	edition	language
2021-04-10	The Journal of Organic Chemistry

https://doi.org/10.1021/acs.joc.0c02993

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