6533b82efe1ef96bd1293162

RESEARCH PRODUCT

Preparation of Samples for Characterization of Arbuscular Mycorrhizal Fungi

Alberto Guillén

subject

0106 biological sciences0301 basic medicinebiologyfungifood and beveragesFungusbiology.organism_classificationIsolation (microbiology)Arbuscular mycorrhizal fungi01 natural sciencesSpore03 medical and health sciences030104 developmental biologyNutrientPropaguleBotanyWet sievingMycelium010606 plant biology & botany

description

Arbuscular mycorrhizal fungi (AMF) are an important element of the plant microbiome as they establish an endosymbiotic relationship with the roots of most plant species. This association enhances access to nutrients and water for plants, and provides the fungus with plant-derived organic carbon. In this chapter, I describe a range of methods to work with AMF including: soil sampling; isolation of AMF propagules (spores, sporocarps, roots, and mycelium) by a wet sieving and centrifugation in a sucrose solution; trap (from field soil with AMF spores) and one-species pot cultures (from AMF spores divided into morphotypes); staining of mycorrhizae in plant roots; and production of diagnostic slides. These methods are widely used in taxonomic and ecological studies to characterize the morphology of AMF.

yearjournalcountryeditionlanguage
2020-11-08
https://doi.org/10.1007/978-1-0716-1040-4_5