6533b82efe1ef96bd1293214

RESEARCH PRODUCT

Electropherotyping of human rotaviruses: An epidemiological survey of rotavirus infections in Sicily

Giuseppe GernaSerenella AristaL. GiovannelliL. TitoneN. Passarani

subject

ElectrophoresisRotavirusSerotypemedicine.medical_specialtyRotavirus RNAeducation.field_of_studyEpidemiologybusiness.industryPopulationInfantRotavirus Infectionsmedicine.disease_causeVirologyRotavirus InfectionsMicrobiologyChild PreschoolRotavirusEpidemiologyHumansRNA ViralMedicineChildbusinesseducationSicily

description

An electrophoretic analysis of rotavirus RNA segments was carried out on 522 faecal specimens, obtained from children hospitalized in Sicily in the period 1981/85. One hundred and one viral isolates could be characterized with respect to the electrophoretic pattern of their genomic RNAs. This analysis revealed that in 1981/82 different electropherotypes cocirculated in the infant population. In 1983 one of the patterns became prevalent; in 1984/85 only one electropherotype was detected, both in Palermo and Catania specimens. The serotyping showed that all viral strains with the prevalent electropherotype were subgroup II and serotype 1. These results contrast with the extensive genome variability of rotavirus strains observed in urban areas.

https://doi.org/10.1007/bf00157019