6533b82efe1ef96bd1293243

RESEARCH PRODUCT

GABAergic projections from the subplate to Cajal-Retzius cells in the neocortex.

Sergei KirischukOlga MyakharPetr Unichenko

subject

Patch-Clamp TechniquesPostsynaptic CurrentNeocortexBiologyInhibitory postsynaptic potentialSynaptic TransmissionMiceSubplateNeural PathwaysmedicineAnimalsgamma-Aminobutyric AcidNeuronsNeocortexGeneral NeuroscienceExcitatory Postsynaptic PotentialsElectric StimulationElectrophysiological PhenomenaMice Inbred C57BLCorticogenesisElectrophysiologymedicine.anatomical_structurenervous systemAnimals NewbornCerebral cortexData Interpretation StatisticalSynapsesGABAergicNeuroscience

description

Subplate neurons and Cajal-Retzius cells play an important role in the corticogenesis. Despite morphological evidence, the question whether subplate neurons innervate Cajal-Retzius cells has not been studied yet. We report that electrical stimulation in the subplate resulted in evoked GABAergic inhibitory postsynaptic currents (eIPSCs) in Cajal-Retzius cells. The eIPSC latency showed minor variability and amounted to approximately 4 ms, suggesting the monosynaptic connection. During the first postnatal week: (i) eIPSC amplitude increased, (ii) eIPSC kinetics sped up, (iii) the size of readily releasable pool increased, and (iv) γ-aminobutyric acid release probability decreased. We conclude that GABAergic subplate neurons innervate Cajal-Retzius cells. Surprisingly, despite the transient nature of both cell populations, these projections show developmental adjustments typical for many nontransient synaptic connections.

yearjournalcountryeditionlanguage
2011-06-14Neuroreport
10.1097/wnr.0b013e32834888a4https://pubmed.ncbi.nlm.nih.gov/21666518