6533b82efe1ef96bd1293469
RESEARCH PRODUCT
Septins 2, 7 and 9 and MAP4 colocalize along the axoneme in the primary cilium and control ciliary length
Marie-christine RouyezMarie-christine RouyezMarie-christine RouyezSophie SaunierSophie SaunierW. James NelsonUwe WolfrumAlexandre BenmerahBenjamin SpitzbarthSerge MostowySerge MostowySerge MostowyRania GhossoubRania GhossoubRania GhossoubQicong HuPascale CossartPascale CossartPascale CossartMarion Faillersubject
AxonemeAxonemeMicrotubule-associated protein[SDV]Life Sciences [q-bio]DIFFUSION BARRIERTUBULINCell Cycle Proteinsmacromolecular substancesORGANIZATIONCYTOSKELETONBiologySeptinMicrotubulesRetinaCell Line03 medical and health sciences0302 clinical medicineMicrotubuleCiliogenesisHumansCiliaCytoskeletonMolecular BiologyAFFINITY-REGULATING KINASEActin030304 developmental biologyCILIOGENESIS0303 health sciencesPrimary ciliumCOMPLEXSperm flagellumCilium030302 biochemistry & molecular biologyColocalizationEpithelial CellsAnatomyCell BiologyActinsCell biology[SDV] Life Sciences [q-bio]MAMMALIAN SEPTINSMAP4CELLSbiological phenomena cell phenomena and immunityMicrotubule-Associated Proteins030217 neurology & neurosurgerySeptinsDevelopmental BiologyResearch Articledescription
International audience; Septins are a large, evolutionarily conserved family of GTPases that form hetero-oligomers and interact with the actin-based cytoskeleton and microtubules. They are involved in scaffolding functions, and form diffusion barriers in budding yeast, the sperm flagellum and the base of primary cilia of kidney epithelial cells. We investigated the role of septins in the primary cilium of retinal pigmented epithelial (RPE) cells, and found that SEPT2 forms a 1:1:1 complex with SEPT7 and SEPT9 and that the three members of this complex colocalize along the length of the axoneme. Similar to observations in kidney epithelial cells, depletion of cilium-localized septins by siRNA-based approaches inhibited ciliogenesis. MAP4, which is a binding partner of SEPT2 and controls the accessibility of septins to microtubules, was also localized to the axoneme where it appeared to negatively regulate ciliary length. Taken together, our data provide new insights into the functions and regulation of septins and MAP4 in the organization of the primary cilium and microtubule-based activities in cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-01-01 |