6533b82efe1ef96bd1293a48

RESEARCH PRODUCT

Préparation de liposomes fonctionnels pour l’encapsulation de composés bioactifs ou de sondes moléculaires fluorescentes pour l’imagerie cellulaire

subject

description

At ICMUB Institute, SUV liposomes have been used to achieve fluorophore encapsulation (subphtalocyanine) for cellular imaging [1] and have been bioconjugated to a peptide (bombesin) to achieve site-specificity, thanks to the structural modification of cholesterol, a component of the liposome, upon introduction of a reactive function. Hence, such liposomes fonctionnalised on the outer face may react with the terminal function of the peptide. Upon purification of the liposome by FPLC a 15 % encapsulation rate was measured by UV/Vis and attempted by ICP. The (outer) diameter of the liposomes (dDLS and dMET) ranged between 20 and 60 nm depending on the type of function or substituents on the outer face of the liposome [2]. Monitoring of the size and stability of the liposomes was achieved over time. This overall approach is also carried out at PAPC team (UMR PAM, AgroSup Dijon) to achieve the the conjugation of the liposome to proteins [3] (b-lactoglobuline) or polysaccharides [4, 5] (chitosan and pectin). The final aim will get functional and stable liposomes in different media. These nano-objects will be sub-mitted to several physical and chemical stress (pH, temperature, oxidation, etc.) and their strength will be analysed. Glutathione will be encapsulated inside the liposomes and used like molecular sensor for the determination of oxidized state medium. These systems will be also choice models for the study of behaviour of cell’s membrane.