6533b82efe1ef96bd1293b66
RESEARCH PRODUCT
Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications
Irina GerasimenkoIrina GerasimenkoJoachim StöckigtY. V. SheludkoY. V. SheludkoMatthias Ungersubject
Indole testTryptamineMonoterpenoid Indole AlkaloidsChromatographybiologyApocynaceaeChemistryPlant ScienceGeneral MedicineIon pairsRhazya strictabiology.organism_classificationBiochemistryHigh-performance liquid chromatographyAnalytical Chemistrychemistry.chemical_compoundComplementary and alternative medicineRauvolfia serpentinaDrug DiscoveryMolecular MedicineFood Sciencedescription
An efficient system for the analysis of indole alkaloids by HPLC on a reversed-phase column using an ion pair technique is described. The optimised chromatographic conditions allowed the successful separation of 22 standard monoterpenoid indole alkaloids (including some isomers) and tryptamine. The described HPLC system was applied to the analysis of alkaloids in intergeneric somatic hybrid cell cultures of Rauvolfia serpentina x Rhazya stricta.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-03-01 | Phytochemical Analysis |