6533b82efe1ef96bd1293f06

RESEARCH PRODUCT

Isolation and characterisation of an isoproturon-mineralisingMethylopilasp. TES from French agricultural soil

Talaat El SebaïGuy SoulasFabrice Martin-laurentBernard Lagacherie

subject

Molecular Sequence DataBiologyDNA RibosomalMicrobiologyEnrichment cultureMicrobiologychemistry.chemical_compoundPlasmidRNA Ribosomal 16SMethylopila sp. TESBotanyGeneticsMolecular BiologySoil MicrobiologyStrain (chemistry)HerbicidesPhenylurea CompoundsAgricultureSequence Analysis DNAMonolinuronBiodegradation16S ribosomal RNAIsolation (microbiology)Bacterial Typing TechniquesCulture MediaBiodegradation EnvironmentalchemistryFranceMethylocystaceae

description

Using enrichment culture three isoproturon (IPU) mineralising bacterial isolates were isolated from a French agricultural soil mineralising up to 50% of the initially added 14C-ring labelled IPU within only eight days. These isolates showed similar metabolic (BIOLOG GN) and amplified rDNA restriction (ARDRA) profiles. Partial 16S rDNA sequencing revealed that they were identical and identified as Methylopila sp TES. This strain harbours a large plasmid (220 kb) putatively bearing essential IPU-degrading genes as demonstrated by a curing experiment. Methylopila sp. TES transformed IPU and its known metabolites to CO2 and biomass but did not degrade chlorotoluron, monolinuron, diuron and linuron.

yearjournalcountryeditionlanguage
2004-06-09FEMS Microbiology Letters
https://doi.org/10.1016/j.femsle.2004.08.017