6533b82ffe1ef96bd129484d
RESEARCH PRODUCT
Native Electrospray-based Metabolomics Enables the Detection of Metal-binding Compounds
Dutton RjDutton RjEckhard ThinesAllegra T. AronAllegra T. AronPieter C. DorresteinPieter C. DorresteinPieter C. DorresteinManuela RaffatelluDaniel PetrasDaniel PetrasDaniel PetrasJulia M. GauglitzJulia M. GauglitzLuis AnteloBüttel IHui ZhiRobin SchmidMalarney KpSaak Ccsubject
0303 health sciencesElectrosprayMetal bindingElectrospray ionization010402 general chemistryMass spectrometry01 natural sciencesCombinatorial chemistrySmall molecule0104 chemical sciencesIon03 medical and health sciencesMetabolomicsMolecule030304 developmental biologydescription
AbstractMetals are essential for the molecular machineries of life, and microbes have evolved a variety of small molecules to acquire, compete for, and utilize metals. Systematic methods for the discovery of metal-small molecule complexes from biological samples are limited. Here we describe a two-step native electrospray ionization mass spectrometry method, in which double-barrel post-column metal-infusion and pH adjustment is combined with ion identity molecular networking, a rule-based informatics workflow. This method can be used to identify metal-binding compounds in complex samples based on defined mass (m/z) offsets of ion features with the same chromatographic profiles. As this native metal metabolomics approach can be easily implemented on any liquid chromatography-based mass spectrometry system, this method has the potential to become a key strategy for elucidating and understanding the role of metal-binding molecules in biology.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-10-30 |