6533b82ffe1ef96bd129504f
RESEARCH PRODUCT
Detecting RNA modifications in the epitranscriptome: predict and validate
Yuri MotorinMark Helmsubject
Epigenomics0301 basic medicineComputational biologyBiologyEpigenesis Genetic03 medical and health sciences0302 clinical medicine[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]EpitranscriptomicsGeneticsAnimalsHumansEpigeneticsRNA Processing Post-TranscriptionalMolecular BiologyComputingMilieux_MISCELLANEOUSGenetics (clinical)GeneticsRNA[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyComputingMethodologies_PATTERNRECOGNITION030104 developmental biologyGene Expression RegulationRNAIdentification (biology)Transcriptome030217 neurology & neurosurgerydescription
RNA modifications are emerging players in the field of post-transcriptional regulation of gene expression, and are attracting a comparable degree of research interest to DNA and histone modifications in the field of epigenetics. We now know of more than 150 RNA modifications and the true potential of a few of these is currently emerging as the consequence of a leap in detection technology, principally associated with high-throughput sequencing. This Review outlines the major developments in this field through a structured discussion of detection principles, lays out advantages and drawbacks of new high-throughput methods and presents conventional biophysical identification of modifications as meaningful ways for validation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-05-01 | Nature Reviews Genetics |