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RESEARCH PRODUCT
Advanced Strategies for Food-Grade Protein Production: A New E. coli/Lactic Acid Bacteria Shuttle Vector for Improved Cloning and Food-Grade Expression
Aldo NicosiaAldo NicosiaMarcello Tagliaviasubject
Microbiology (medical)Lactococcusfood-grade expression vectorsBiologyMicrobiologylaw.inventionMetabolic engineering03 medical and health sciencesShuttle vectorresistance cassette removallawVirologyProtein purificationlcsh:QH301-705.5Gene030304 developmental biology0303 health sciencesExpression vector030306 microbiologyfood and beveragesbiology.organism_classificationgenerally recognized as safe (GRAS) microorganismsshuttle expression vectorslcsh:Biology (General)BiochemistryRecombinant DNAadvanced food-grade cloning: flippase (FLP) recombinaselactic acid bacteria (LAB)Bacteriadescription
Food-grade production of recombinant proteins in Gram-positive bacteria, especially in LAB (i.e., Lactococcus, Lactobacillus, and Streptococcus), is of great interest in the areas of recombinant enzyme production, industrial food fermentation, gene and metabolic engineering, as well as antigen delivery for oral vaccination. Food-grade expression relies on hosts generally considered as safe organisms and on clone selection not dependent on antibiotic markers, which limit the overall DNA manipulation workflow, as it can be carried out only in the expression host and not in E. coli. Moreover, many commercial expression vectors lack useful elements for protein purification. We constructed a &ldquo
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-04-27 | Microorganisms |